Structure Elucidation of a Novel Pentasaccharide Meeniosefrom Babalus bubalis Colostrum

Oligosaccharides present in the milk of different origin have shown varied biological activities. Oligosaccharide isolated from Buffalo milk has shown encouraging immune stimulant activities. With a view to isolate more biologically active oligosaccharides from colostrum of buffalo (Babalus bubalis). Colostrum was collected and processed by modified method of Kobata and Ginsberg, involving deproteination, centrifugation and lyophilization followed by acetylation and silica gel column chromatography of derivatized oligosaccharides. The structure of isolated penta saccharide was elucidated by chemical transformation, chemical degradation, H NMR, C NMR, 2D NMR (COSY, TOCSY and HSQC) and mass spectrometry. The geometry of isolated compound Meeniose was optimized at B3LYP method and 6-311+G (d, p) basis set. In the light of results obtained from above experiments, the structure of novel pentasaccharides meeniose was confirmed as β-GalNAc (1→2)β-Gal(1→4)β-GlcNAc (1→3) -β-GalNAc (1→4)Glc.